PI3K p85/p55 antibody (pTyr199, pTyr467)

Catalog No. ABIN7207993

Product Details anti-PI3K p85/p55 Antibody

Target: PI3K p85/p55 (Phosphoinositide 3 Kinase, p85/p55 (PI3K p85/p55))

Binding Specificity: pTyr199, pTyr467

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI3K p85/p55 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody

Specificity: Phospho-PI 3-kinase p85/p55 (Y467/199) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85/p55 protein only when phosphorylated at Y467/199.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PI 3-kinase p85/p55 Phospho-Tyr467/199

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PI3K p85/p55

Target: PI3K p85/p55 (Phosphoinositide 3 Kinase, p85/p55 (PI3K p85/p55))

Alternative Name: PI 3-kinase p85/p55 (PI3K p85/p55 Products)

Background: Rabbit Anti-PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody,PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph,Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by PIK3CG (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma) is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for PIK3CG.,Phosphatidylinositol 3-kinase regulatory subunit alpha

Gene ID: 5295