MLKL antibody (pSer358)

Catalog No. ABIN7212282

Product Details anti-MLKL Antibody

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Binding Specificity: pSer358

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLKL antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purpose: phospho-MLKL (S358) Mouse Monoclonal Antibody (6F8)

Specificity: Phospho-MLKL (S358) protein detects endogenous levels of MLKL.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Immunogen: Synthetic Peptide of phospho-MLKL (S358)

Clone: 6F8

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:200).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MLKL

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Alternative Name: MLKL (MLKL Products)

Synonyms: MLKL antibody, 9130019I15Rik antibody, mixed lineage kinase domain like pseudokinase antibody, mixed lineage kinase domain-like antibody, MLKL antibody, mlkl antibody, Mlkl antibody

Background: Mouse Anti-phospho-MLKL (S358) Mouse Monoclonal Antibody (6F8),MLKL,MLKL (mixed lineage kinase domain like) belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain, however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF) -induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of MLKL inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for MLKL.,MLKL

Molecular Weight: observerd band 54kDa

Gene ID: 197259

UniProt: Q8NB16