MEK1/2 antibody (pSer218, pSer222)

Catalog No. ABIN7213034

Product Details anti-MEK1/2 Antibody

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Binding Specificity: pSer218, pSer222

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1/2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: MEK-1/2 (phospho Ser218/222) Polyclonal Antibody

Specificity: Phospho-MEK-1/2 (S218/222) Polyclonal Antibody detects endogenous levels of MEK-1/2 protein only when phosphorylated at S218/222.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human MEK-1/2 Phospho-Ser218/222

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MEK1/2

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Alternative Name: MEK-1/2 (MAP2K1/2 Products)

Background: Rabbit Anti-MEK-1/2 (phospho Ser218/222) Polyclonal Antibody,MAP2K1, MEK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K2, MEK2, MKK2, PRKMK2, Dual specificity mitogen-activated protein k,Mitogen-activated protein kinase 1 encoded by MAP2K1 is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. Mitogen-activated protein kinase 1 lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway,Mitogen-activated protein kinase 1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.,Dual specificity mitogen-activated protein kinase kinase 1

Molecular Weight: observerd band 48kDa

Gene ID: 5604, 5605