Luciferase antibody

Catalog No. ABIN7212242

Product Details anti-Luciferase Antibody

Target: Luciferase

Reactivity: Firefly

Host: Mouse

Clonality: Monoclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Luciferase Mouse Monoclonal Antibody (6B8)

Specificity: Luciferase protein detects endogenous levels of Luciferase.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Immunogen: Recombinant Protein of Luciferase of Luciferase

Clone: 6B8

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:100-1:200).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Luciferase

Target: Luciferase

Abstract: Luciferase Products

Synonyms: hypothetical protein antibody, luciferase antibody, C25G6.3 antibody, Gbro_2186 antibody

Background: Mouse Anti-Luciferase Mouse Monoclonal Antibody (6B8),Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.,Luciferase

Molecular Weight: observerd band 60kDa

UniProt: P08659