EIF2A antibody (pSer51)

Catalog No. ABIN7203754

Product Details anti-EIF2A Antibody

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Binding Specificity: pSer51

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: eIF2α (phospho Ser51) Polyclonal Antibody

Specificity: Phospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human eIF2alpha Phospho-Ser51

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: eIF2alpha (EIF2A Products)

Synonyms: ATEIF2-A2 antibody, EIF2-A2 antibody, eukaryotic translation initiation factor 2 alpha subunit antibody, EIF-2A antibody, MST089 antibody, MSTP004 antibody, MSTP089 antibody, D030048D22 antibody, D3Ertd194e antibody, im:7145971 antibody, zgc:110460 antibody, DDBDRAFT_0185936 antibody, DDBDRAFT_0234177 antibody, DDB_0185936 antibody, DDB_0234177 antibody, Eif2a antibody, eif2a antibody, eif2alpha antibody, EIF2A antibody, CG9946 antibody, Dmel\\CG9946 antibody, Eif2alpha antibody, IF2A_DROME antibody, M(1)14C antibody, M(1)19-153 antibody, M(1)8e3-10 antibody, deIF2alpha antibody, eIF-2 antibody, eIF-2 a antibody, eIF-2 alpha antibody, eIF2 alpha antibody, eIF2-alpha antibody, eIF2a antibody, eIF2alpha antibody, eiF-2alpha antibody, elF2alpha antibody, l(1)14Cf antibody, l(1)19-153 antibody, eukaryotic translation initiation factor 2 alpha subunit antibody, eukaryotic translation initiation factor 2A antibody, eukaryotic translation initiation factor 2A, 65kDa antibody, eukaryotic translation initiation factor 2A S homeolog antibody, eukaryotic translation initiation factor antibody, eukaryotic translation Initiation Factor 2alpha antibody, EIF2 ALPHA antibody, EIF2A antibody, Eif2a antibody, eif2a antibody, eIF2a antibody, eif2a.S antibody, eif2A antibody, eIF-2alpha antibody

Background: Rabbit Anti-eIF2α (phospho Ser51) Polyclonal Antibody,EIF2S1, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha,The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2, MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).,Eukaryotic translation initiation factor 2 subunit 1

Gene ID: 1965

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly