CDH6 antibody (AA 281-380)

Catalog No. ABIN715286

Product Details anti-CDH6 Antibody

Target: CDH6 (K-Cadherin (CDH6))

Binding Specificity: AA 281-380

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDH6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human K Cadherin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100

Restrictions: For Research Use only

Independent Validation (FACS)

Validation #029743 (Flow Cytometry)

by: Flow Cytometry & Cell Separation Facility, Purdue University

No.: #029743

Date: 07/01/2014

Lot Number: 130823

Method validated: Flow Cytometry

Positive Control: 786-O cells and [MCF-7 cells] (http://www.nature.com/bjc/journal/v90/n5/fig_tab/6601640f10.html#figure-title)

Notes: As expected, strong signal was observed in the cells stained with anti-K-Cadherin (CDH6) antibody. A small amount of non-specific staining was observed from the isotype and secondary antibody only negative controls compared with unstained cells.

Validation Images

Figure 1. Histogram of 786-O cells stained with anti-K-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).

Figure 2. Histogram of MCF-7 cells stained with anti-K-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).

Full Methods

Primary Antibody:

• Antigen: K-Cadherin (CDH6) antibody
• Catalog number: ABIN715286
• Lot number: 130823

Secondary Antibody:

• Antibody: Goat anti-rabbit IgG-Alexa 647

Full Protocol:

• Positive control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
• Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - 1 µg primary antibody - 1 µg isotype control antibody
• Cells were incubated for 30 min on ice.
• Labeled cells were washed twice in PBS containing 0.5% BSA.
• Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
• Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
• Labeled cells were washed twice in PBS containing 0.5% BSA.
• Propidium Iodide (PI) was added to discern live cells from dead cells.
• Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).

Experimental Notes: - The data displayed is gated on PI negative cells.

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CDH6

Target: CDH6 (K-Cadherin (CDH6))

Alternative Name: K Cadherin (CDH6 Products)

Synonyms: KCAD antibody, K-cadherin antibody, cad6 antibody, cadherin-6 antibody, CAD6 antibody, c-cad6B antibody, cadherin 6 antibody, Cdh6 antibody, CDH6 antibody

Background:

Synonyms: CAD6, KCAD, Cadherin-6, Kidney cadherin, K-cadherin, CDH6

Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.

Gene ID: 1004

UniProt: P55285

Pathways: Cell-Cell Junction Organization