USP28 antibody (AA 101-200) (Biotin)

Catalog No. ABIN715243

Product Details anti-USP28 Antibody (Biotin)

Target: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Binding Specificity: AA 101-200

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP28 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human USP28

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for USP28

Target: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Alternative Name: USP28 (USP28 Products)

Synonyms: im:7138195 antibody, USP28 antibody, 9830148O20Rik antibody, AU022237 antibody, mKIAA1515 antibody, ubiquitin specific peptidase 28 antibody, USP28 antibody, usp28 antibody, Usp28 antibody

Background:

Synonyms: Deubiquitinating enzyme 28, KIAA1515, Ubiquitin carboxyl terminal hydrolase 28, Ubiquitin carboxyl-terminal hydrolase 28, Ubiquitin specic peptidase 28, Ubiquitin specic processing protease 28, Ubiquitin specic protease 28, Ubiquitin thioesterase 28, Ubiquitin thiolesterase 28, Ubiquitin-specic-processing protease 28, UBP28_HUMAN, USP 28, USP28, USP28 protein, USP-28.

Background: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.

Gene ID: 57646