MYLK antibody (AA 1375-1524)

Catalog No. ABIN7193606

Product Details anti-MYLK Antibody

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Binding Specificity: AA 1375-1524

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYLK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: MYLK Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human MYLK (AA:1375-1524) expressed in E. Coli.

Clone: 7F2B11

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200-1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for MYLK

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Alternative Name: MYLK (MYLK Products)

Background:

Description:

This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.

Aliases: KRP, AAT7, MLCK, MLCK1, MMIHS, MYLK1, s mMLCK, MLCK108, MLCK210, MSTP083

Molecular Weight: 210kDa

Gene ID: 4638

UniProt: Q15746