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Vimentin antibody (AA 2-466)

Name: Vimentin antibody (AA 2-466)
Brand: antibodies-online
SKU: ABIN7193582
Price: 511.5 USD
Availability: InStock

VIM Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

Western blot analysis using VIM mAb against HEK293 (1) and VIM (AA: 2-466)-hIgGFc transfected HEK293 (2) cell lysate. (Vimentin antibody (AA 2-466))

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using VIM mouse mAb with DAB staining. (Vimentin antibody (AA 2-466))

IHC

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (Vimentin antibody (AA 2-466))

ELISA

Western blot analysis using VIM mAb against human VIM (AA: 2-466) recombinant protein. (Vimentin antibody (AA 2-466))

Western blot analysis using VIM mouse mAb against Jurkat (1), K562 (2), SK-N-SH (3), SH-SY5Y (4), Hela (5), NIH/3T3 (6), C6 (7), and RAW264. (Vimentin antibody (AA 2-466))

Flow cytometric analysis of Hela cells using VIM mouse mAb (green) and negative control (red). (Vimentin antibody (AA 2-466))

FACS

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using VIM mouse mAb with DAB staining. (Vimentin antibody (AA 2-466))

IHC

7 images

Catalog No. ABIN7193582

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Binding Specificity
32

18

16

15

15

15

11

9

7

7

4

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-466
Reactivity
341

133

130

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human
Host
195

129

11

6

6

5

2
Mouse
Compatible Secondaries
Clonality
211

142
Monoclonal
Conjugate
203

19

14

14

10

7

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
285

129

105

99

96

64

46

40

39

33

23

19

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Purpose

Vimentin Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human VIM (AA: 2-466) expressed in E. Coli.

Clone

3A1F2

Isotype

IgG2a

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anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, StM Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated

Application Notes

ELISA: 1/10000
FCM: 1/200-1/400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Target

Vimentin (VIM)

Alternative Name

Vimentin (VIM Products)

Synonyms

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

Background

Description:
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Aliases: FLJ36605, VIM

Molecular Weight

53.7kDa

Gene ID

7431

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis