MLH1 antibody (AA 381-483)

Catalog No. ABIN7193525

Product Details anti-MLH1 Antibody

Target: MLH1 (MutL Homolog 1 (MLH1))

Binding Specificity: AA 381-483

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: MLH1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human MLH1 (AA: 381-483) expressed in E. Coli.

Clone: 8C12B7

Isotype: IgG2b

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MLH1 (MLH1 Products)

Background:

Description:

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).

Aliases: FCC2, COCA2, HNPCC, hMLH1, HNPCC2

Molecular Weight: 84.6kDa

Gene ID: 4292

UniProt: P40692

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response