Calreticulin antibody (AA 18-417)

Catalog No. ABIN7193510

Product Details anti-Calreticulin Antibody

Target: Calreticulin (CALR)

Binding Specificity: AA 18-417

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purification: purified

Immunogen: Purified recombinant fragment of human CALR (AA: 18-417) expressed in E. coli.

Clone: 7B3D7

Isotype: IgG2a

Application Details

Application Notes: WB:1:500 - 1:2000, IHC:1:200 - 1:1000, FCM:1:200 - 1:400, ELISA:1:10000,

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: CALR (CALR Products)

Synonyms: CRT antibody, RO antibody, SSA antibody, cC1qR antibody, crc antibody, crt antibody, Calregulin antibody, CG9429 antibody, Clr antibody, Dmel\\CG9429 antibody, anon-EST:Liang-2.11 antibody, anon-EST:Liang-2.17 antibody, anon-EST:fe3D7 antibody, clone 2.11 antibody, clone 2.17 antibody, eth antibody, potp antibody, cc1qr antibody, ssa antibody, NV19007 antibody, CRP55 antibody, ERp60 antibody, HACBP antibody, CHOT1 antibody, CHT1 antibody, CT1 antibody, AA589632 antibody, CRTR antibody, Creat antibody, CCDS1 antibody, calrl2 antibody, fc66d10 antibody, wu:fc66d10 antibody, wu:fj50c03 antibody, zgc:66153 antibody, calreticulin antibody, calreticulin L homeolog antibody, Calreticulin antibody, putative calreticulin antibody, solute carrier family 6 member 8 antibody, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 antibody, CALR antibody, calr.L antibody, Crt antibody, crt antibody, CRT antibody, Calr antibody, calr antibody, LMJF_31_2600 antibody, Crc antibody, Slc6a8 antibody, SLC6A8 antibody, LOC8269812 antibody

Background:

Description: Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Aliases: RO, CRT, SSA, cC1qR, HEL-S-99n

Molecular Weight: 48.1 kDa

Gene ID: 811

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response