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POLA1 antibody (N-Term)

POLA1 Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7184445
  • Target See all POLA1 Antibodies
    POLA1 (DNA Polymerase alpha (POLA1))
    Binding Specificity
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 31
    • 12
    • 11
    • 8
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 39
    • 5
    Rabbit
    Clonality
    • 40
    • 4
    Polyclonal
    Conjugate
    • 20
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    This POLA1 antibody is un-conjugated
    Application
    • 19
    • 11
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Cross-Reactivity
    Human
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    Immunogen
    Synthesized peptide derived from N-terminal of Human DNA Polymerase alpha.
    Isotype
    IgG
    Top Product
    Discover our top product POLA1 Primary Antibody
  • Application Notes
    WB:1:500-1:3000,
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C,-80 °C
    Storage Comment
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • Target
    POLA1 (DNA Polymerase alpha (POLA1))
    Alternative Name
    POLA1 (POLA1 Products)
    Synonyms
    pola antibody, AW321876 antibody, Pola antibody, POLA antibody, p180 antibody, polymerase (DNA directed), alpha 1, catalytic subunit S homeolog antibody, polymerase (DNA directed), alpha 1 antibody, DNA polymerase alpha 1, catalytic subunit antibody, pola1.S antibody, Pola1 antibody, POLA1 antibody
    Background

    Background: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

    Wong S.W., EMBO J. 7:37-47(1988).
    Pearson B.E., Mol. Cell. Biol. 11:2081-2095(1991).
    Hsi K.-L., Nucleic Acids Res. 18:6231-6237(1990).

    Aliases: DKFZp686K1672 antibody, DNA polymerase alpha 1 catalytic subunit antibody, DNA polymerase alpha catalytic subunit antibody, DNA polymerase alpha catalytic subunit p180 antibody, DNA polymerase alpha p180 subunit antibody, DPOLA_HUMAN antibody, OTTHUMP00000023089 antibody, OTTHUMP00000023090 antibody, P180 antibody, p180 subunit antibody, POLA antibody, POLA1 antibody, Polymerase (DNA directed), alpha 1, catalytic subunit antibody, Polymerase (DNA-directed), alpha (70kD) antibody

    UniProt
    P09884
    Pathways
    SARS-CoV-2 Protein Interactome
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