POLR3B antibody

Catalog No. ABIN7184076

Product Details anti-POLR3B Antibody

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Human, Mouse

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from internal of Human RPC2.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:3000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for POLR3B

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Alternative Name: POLR3B (POLR3B Products)

Synonyms: RGD1565311 antibody, C128 antibody, HLD8 antibody, RPC2 antibody, 2700078H01Rik antibody, A330032P03Rik antibody, C85372 antibody, RNA polymerase III subunit B antibody, polymerase (RNA) III (DNA directed) polypeptide B antibody, Polr3b antibody, POLR3B antibody

Background:

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Hu P., Mol. Cell. Biol. 22:8044-8055(2002).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Ota T., Nat. Genet. 36:40-45(2004).

Aliases: C128 antibody, DNA directed RNA polymerase III 127.6 kDa polypeptide antibody, DNA directed RNA polymerase III subunit B antibody, DNA-directed RNA polymerase III 127.6 kDa polypeptide antibody, DNA-directed RNA polymerase III subunit B antibody, DNA-directed RNA polymerase III subunit RPC2 antibody, FLJ10388 antibody, polr3b antibody, Polymerase (RNA) III (DNA directed) polypeptide B antibody, RNA polymerase III subunit C2 antibody, RNA polymerase III subunit RPC2 antibody, RPC2 antibody, RPC2_HUMAN antibody

UniProt: Q9NW08