CKAP5 antibody (AA 81-180)

Catalog No. ABIN714011

Product Details anti-CKAP5 Antibody

Target: CKAP5 (Cytoskeleton Associated Protein 5 (CKAP5))

Binding Specificity: AA 81-180

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKAP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CKAP5/ch TOG

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CKAP5

Target: CKAP5 (Cytoskeleton Associated Protein 5 (CKAP5))

Alternative Name: CKAP5 (CKAP5 Products)

Background:

Synonyms: Ch TOG protein, chTOG, CHTOG protein, CKAP5_HUMAN, CKAP 5, CKAP5, CKAP5 protein, Colonic and hepatic tumor over expressed, Colonic and hepatic tumor over expressed protein, Colonic and hepatic tumor overexpressed protein, Cytoskeleton associated protein 5, FLJ35359, KIAA0097, TOG, TOGp.

Background: CKAP5 is a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation, it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified.

Gene ID: 9793

Pathways: M Phase