NEK2 antibody (AA 1-100)

Catalog No. ABIN713921

Product Details anti-NEK2 Antibody

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Binding Specificity: AA 1-100

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NEK2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NEK2 antibody (ABIN713921)

Zeng, Han, Wang, Cai, Huang, Luo, Liu, Zhuo, Dai, Zhao, Liang, Zhong: "Overexpression of NIMA-related kinase 2 is associated with progression and poor prognosis of prostate cancer." in: BMC urology, Vol. 15, pp. 90, (2015) (PubMed).

Zhong, Guan, Dong, Yang, Liu, Zhang: "Examining Nek2 as a better proliferation marker in non-small cell lung cancer prognosis." in: Tumour biology, Vol. 35, Issue 7, pp. 7155-62, (2014) (PubMed).

Zhong, Guan, Liu, Zhang: "Aberrant expression of NEK2 and its clinical significance in non-small cell lung cancer." in: Oncology letters, Vol. 8, Issue 4, pp. 1470-1476, (2014) (PubMed).

Target Details for NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Alternative Name: NEK2 (NEK2 Products)

Synonyms: AA617254 antibody, C77054 antibody, HsPK21 antibody, NEK2A antibody, NLK1 antibody, ATNEK2 antibody, NIMA-RELATED KINASE2 antibody, NIMA-related kinase 2 antibody, T9J14.24 antibody, T9J14_24 antibody, NIMA related kinase 2 antibody, NIMA (never in mitosis gene a)-related expressed kinase 2 antibody, NIMA-related kinase 2 antibody, NEK2 antibody, Nek2 antibody

Background:

Synonyms: NLK1, RP67, NEK2A, HsPK21, PPP1R111, Serine/threonine-protein kinase Nek2, HSPK 21, Never in mitosis A-related kinase 2, NimA-related protein kinase 2, NimA-like protein kinase 1, NEK2

Background: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP25 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC8. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC2 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC, SGOL1, NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.

Gene ID: 4751

UniProt: P51955

Pathways: M Phase