NDC80 antibody (AA 21-120)

Catalog No. ABIN713846

Product Details anti-NDC80 Antibody

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Binding Specificity: AA 21-120

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HEC1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: HEC1 (NDC80 Products)

Background:

Synonyms: HEC 1, HEC, Highly Expressed in Cancer, Highly expressed in cancer protein, Highly expressed in cancer rich in leucine heptad repeats, HsHec1, hsNDC80, Kinetochore Associated 2, Kinetochore associated protein 2, Kinetochore protein Hec1, Kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, KNTC 2, KNTC2, NDC 80, NDC80, NDC80_HUMAN, Retinoblastoma associated protein HEC, Retinoblastoma-associated protein HEC.

Background: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

Gene ID: 10403

Pathways: Maintenance of Protein Location