Topoisomerase II alpha antibody (Thr1343)

Catalog No. ABIN7180100

Product Details anti-Topoisomerase II alpha Antibody

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Binding Specificity: Thr1343

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Human

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from Human TOP2A around the phosphorylation site of threonine 1343 (E-K-T(p)-D-D).

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:3000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A (TOP2A Products)

Synonyms: TOP2 antibody, TP2A antibody, TOP2A antibody, Top-2 antibody, TOP-2 antibody, F12B17.110 antibody, F12B17_110 antibody, thimet metalloendopeptidase 2 antibody, fc08c07 antibody, wu:fc08c07 antibody, DNA topoisomerase II alpha antibody, DNA topoisomerase II, alpha isozyme antibody, topoisomerase (DNA) II alpha antibody, Zincin-like metalloproteases family protein antibody, TOP2A antibody, Bm1_08705 antibody, Top2a antibody, AT5G10540 antibody, top2a antibody

Background:

Background: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988).
Wasserman R.A., Cancer Res. 53:3591-3596(1993).
Lang A.J., Gene 221:255-266(1998).

Aliases: alpha isozyme antibody, ATP hydrolyzing DNA topoisomerase II alfa antibody, DNA gyrase antibody, DNA topoisomerase (ATP hydrolyzing) antibody, DNA topoisomerase 2 alpha antibody, DNA topoisomerase 2-alpha antibody, DNA topoisomerase II 170 kD antibody, DNA topoisomerase II alpha isozyme antibody, DNA topoisomerase II antibody, DNA Topoisomerase2 antibody, TOP 2A antibody, TOP2 antibody, TOP2A antibody, TOP2A_HUMAN antibody, Topoisomerase DNA II alpha 170 kDa antibody, TP2A antibody

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases