APLF antibody (Ser116)

Catalog No. ABIN7179974

Product Details anti-APLF Antibody

Target: APLF (Aprataxin and PNKP Like Factor (APLF))

Binding Specificity: Ser116

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APLF antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity: Human, Mouse

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from Human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V).

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:3000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for APLF

Target: APLF (Aprataxin and PNKP Like Factor (APLF))

Alternative Name: APLF (APLF Products)

Background:

Background: Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.

Saunders W.S., J. Cell Sci. 104:573-582(1993).
Ye Q., J. Biol. Chem. 271:14653-14656(1996).
Ye Q., J. Biol. Chem. 272:14983-14989(1997).

Aliases: 2010301N04Rik antibody, AI452191 antibody, Aplf antibody, APLF_HUMAN antibody, Aprataxin and PNK-like factor antibody, Apurinic-apyrimidinic endonuclease APLF antibody, C2orf13 antibody, PNK and APTX like FHA protein antibody, PNK and APTX-like FHA domain-containing protein antibody, RGD1565557 antibody, XIP1 antibody, XRCC1 interacting protein 1 antibody, XRCC1-interacting protein 1 antibody

UniProt: Q8IW19

Pathways: DNA Damage Repair