WDR61 antibody (AA 2-305)

Catalog No. ABIN7175778

Product Details anti-WDR61 Antibody

Target: WDR61 (WD Repeat Domain 61 (WDR61))

Binding Specificity: AA 2-305

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WDR61 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human, Mouse

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human WD repeat-containing protein 61 protein (2-305AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for WDR61

Target: WDR61 (WD Repeat Domain 61 (WDR61))

Alternative Name: WDR61 (WDR61 Products)

Synonyms: WDR61 antibody, REC14 antibody, SKI8 antibody, 2700038L12Rik antibody, 2810418I05Rik antibody, RGD1308228 antibody, WD repeat domain 61 antibody, Ski complex subunit Rec14 antibody, WD repeat domain 61 L homeolog antibody, wdr61 antibody, WDR61 antibody, LOC692852 antibody, LOC732907 antibody, rec14 antibody, Wdr61 antibody, wdr61.L antibody

Background:

Background: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and \\\'Ser-2\\\'- and \\\'Ser-5\\\'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 \\\'Lys-4\\\' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of \\\'Lys-120\\\' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3\\\' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 \\\'Lys-4\\\' (H3K4me3), dimethylation on histone H3 \\\'Lys-79\\\' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.

Aliases: Meiotic recombination REC14 protein homolog antibody, REC14 antibody, Recombination protein REC14 antibody, Ski8 antibody, SKI8 homolog antibody, WD repeat containing protein 61 antibody, WD repeat domain 61 antibody, WD repeat-containing protein 61 antibody, WDR 61 antibody, wdr61 antibody, WDR61_HUMAN antibody

UniProt: Q9GZS3