USP28 antibody (AA 430-580)

Catalog No. ABIN7174302

Product Details anti-USP28 Antibody

Target: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Binding Specificity: AA 430-580

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP28 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Ubiquitin carboxyl-terminal hydrolase 28 protein (430-580AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for USP28

Target: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Alternative Name: USP28 (USP28 Products)

Synonyms: im:7138195 antibody, USP28 antibody, 9830148O20Rik antibody, AU022237 antibody, mKIAA1515 antibody, ubiquitin specific peptidase 28 antibody, USP28 antibody, usp28 antibody, Usp28 antibody

Background:

Background: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).

Aliases: Deubiquitinating enzyme 28 antibody, KIAA1515 antibody, Ubiquitin carboxyl terminal hydrolase 28 antibody, Ubiquitin carboxyl-terminal hydrolase 28 antibody, ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody, Ubiquitin specific peptidase 28 antibody, Ubiquitin specific processing protease 28 antibody, Ubiquitin specific protease 28 antibody, Ubiquitin thioesterase 28 antibody, Ubiquitin thiolesterase 28 antibody, Ubiquitin-specific-processing protease 28 antibody, UBP28_HUMAN antibody, USP 28 antibody, USP28 antibody, USP28 protein antibody

UniProt: Q96RU2