USP1 antibody (AA 453-592)

Catalog No. ABIN7174250

Product Details anti-USP1 Antibody

Target: USP1 (Ubiquitin Specific Peptidase 1 (USP1))

Binding Specificity: AA 453-592

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Cross-Reactivity: Human, Rat

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Ubiquitin carboxyl-terminal hydrolase 10 protein (453-592AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for USP1

Target: USP1 (Ubiquitin Specific Peptidase 1 (USP1))

Alternative Name: USP1 (USP1 Products)

Synonyms: usp1-a antibody, UBP antibody, ubiquitin specific peptidase 1 antibody, ubiquitin specific peptidase 1 S homeolog antibody, USP1 antibody, usp1.S antibody, Usp1 antibody

Background:

Background: Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989).

Aliases: Deubiquitinating enzyme 10 antibody, KIAA0190 antibody, MGC124997 antibody, MGC2621 antibody, Ubiquitin carboxyl terminal hydrolase 10 antibody, Ubiquitin carboxyl-terminal hydrolase 10 antibody, Ubiquitin specific peptidase 10 antibody, Ubiquitin specific processing protease 10 antibody, Ubiquitin specific protease 10 antibody, Ubiquitin thioesterase 10 antibody, Ubiquitin thiolesterase 10 antibody, Ubiquitin-specific-processing protease 10 antibody, UBP10_HUMAN antibody, UBPO antibody, USP 10 antibody, USP10 antibody

UniProt: Q14694