YAP1 antibody (AA 155-504)

Catalog No. ABIN7172751

Product Details anti-YAP1 Antibody

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Binding Specificity: AA 155-504

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Transcriptional coactivator YAP1 protein (155-504AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IF:1:100-1:500,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP1 (YAP1 Products)

Background:

Background: Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).

Aliases: 65 kDa Yes associated protein antibody, 65 kDa Yes-associated protein antibody, COB1 antibody, YAp 1 antibody, YAP 65 antibody, YAP antibody, YAP-1 antibody, YAP1 antibody, YAP1_HUMAN antibody, YAP2 antibody, YAP65 antibody, yes -associated protein delta antibody, Yes associated protein 1 65 kDa antibody, Yes associated protein 1 antibody, Yes associated protein 2 antibody, yes associated protein beta antibody, YKI antibody, Yorkie homolog antibody

UniProt: P46937

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha