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PD-L1 antibody (AA 19-238)

Name: PD-L1 antibody (AA 19-238)
Brand: antibodies-online
SKU: ABIN7164674
Price: 385 USD
Availability: InStock

PD-L1 Reactivity: Human WB, FACS, IHC, ELISA, IF Host: Mouse Monoclonal 9B5F4 unconjugated

Western Blot Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate All lanes: PD-L1 antibody at 1:2500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 33 kDa Observed band size: 55 kDa (PD-L1 antibody (AA 19-238))

Overlay histogram showing A549 cells stained with ABIN7164674 (red line) at 1:150. (PD-L1 antibody (AA 19-238))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate All lanes: PD-L1 antibody at 1:1000, 1:2000, 1:4000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 33 kDa Observed band size: 55 kDa (PD-L1 antibody (AA 19-238))

IHC image of ABIN7164674 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (PD-L1 antibody (AA 19-238))

IHC

IHC image of ABIN7164674 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. (PD-L1 antibody (AA 19-238))

IHC

Immunofluorescence staining of 293 cells with ABIN7164674 at 1:100, counter-stained with DAPI. (PD-L1 antibody (AA 19-238))

Immunofluorescence staining of A549 cells with ABIN7164674 at 1:100, counter-stained with DAPI. (PD-L1 antibody (AA 19-238))

Immunofluorescence staining of Hela cells with ABIN7164674 at 1:100, counter-stained with DAPI. (PD-L1 antibody (AA 19-238))

Overlay histogram showing 293 cells stained with ABIN7164674 (red line) at 1:150. (PD-L1 antibody (AA 19-238))

FACS

9 images

Catalog No. ABIN7164674

Datasheet as PDF

Target See all PD-L1 Antibodies

PD-L1 (CD274 (PD-L1))
Binding Specificity
29

16

15

13

11

9

7

7

5

5

4

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 19-238
Reactivity
165

114

62

6

5

5

5

1

1
Human
Host
131

53

19

4

2

1
Mouse
Compatible Secondaries
Clonality
113

95

2
Monoclonal
Conjugate
91

15

14

11

8

5

4

4

4

4

4

4

4

4

4

4

4

3

3

2

2

2

2

2

2

2

1

1
This PD-L1 antibody is un-conjugated
Application
132

102

55

40

35

28

17

16

13

12

10

7

6

4

3

3

2

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Cross-Reactivity

Human

Purification

Protein G purified

Immunogen

Recombinant Human Programmed cell death 1 ligand 1 protein (19-238AA)

Clone

9B5F4

Isotype

IgG2b

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Verified PD-L1 Reactivity: Human WB, FACS, ELISA, IF Host: Goat Polyclonal unconjugated

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PD-L1 Reactivity: Human FACS, IHC, ELISA Host: Mouse Monoclonal 6D9A2 unconjugated

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PD-L1 Reactivity: Human, Mouse WB, FACS, IHC, ELISA, IF, Coat, StM Host: Mouse Monoclonal PDL1-2746 unconjugated

anti-CD274 (PD-L1) (AA 48-290) antibody
PD-L1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD274 (PD-L1) (Extracellular Domain) antibody
PD-L1 Reactivity: Human IHC Host: Rabbit Monoclonal unconjugated

anti-CD274 (PD-L1) (AA 34-241) antibody
PD-L1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 24-153) antibody
PD-L1 Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 7D2A10 unconjugated

anti-CD274 (PD-L1) (C-Term) antibody
KO Validated PD-L1 Reactivity: Human WB, FACS, ICC, IF, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 19-238) antibody
PD-L1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 201-290) antibody
PD-L1 Reactivity: Mouse, Rat WB, ELISA, IF (cc), IHC (p), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Application Notes

Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:100-1:300,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

PD-L1 (CD274 (PD-L1))

Alternative Name

PD-L1 (PD-L1 Products)

Synonyms

B7-H antibody, B7H1 antibody, PD-L1 antibody, PDCD1L1 antibody, PDCD1LG1 antibody, PDL1 antibody, A530045L16Rik antibody, B7h1 antibody, Pdcd1l1 antibody, Pdcd1lg1 antibody, Pdl1 antibody, RGD1566211 antibody, CD274 molecule antibody, CD274 antigen antibody, programmed cell death 1 ligand 2 antibody, CD274 antibody, Cd274 antibody, PDCD1LG2 antibody

Background

Background: Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077)
Aliases: Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274), CD274, B7H1 PDCD1L1 PDCD1LG1 PDL1

UniProt

Q9NZQ7

Pathways

Cancer Immune Checkpoints