PKM antibody

Catalog No. ABIN7163482

Product Details anti-PKM Antibody

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKM antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Cross-Reactivity: Human

Purification: Affinity purified

Immunogen: Recombinant human PKM2 protein

Isotype: IgG

Application Details

Application Notes: Recommended dilution:WB:1:500-1:5000,IP:1:200-1:2000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1.0 mg/mL in phosphate buffer without Mg2+/Ca2+, pH 7.4, 150 mM NaCl and 50 % glycerol

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: PKM (PKM Products)

Synonyms: CTHBP antibody, OIP3 antibody, PK3 antibody, PKM2 antibody, TCB antibody, THBP1 antibody, AA414905 antibody, AL024370 antibody, AL024424 antibody, Pk-2 antibody, Pk-3 antibody, Pk3 antibody, Pkm2 antibody, PKM12 antibody, CG7070 antibody, Dmel\\CG7070 antibody, PYK antibody, Pyk antibody, cb1029 antibody, fj33f03 antibody, pkm2 antibody, pkm2a antibody, wu:fj33f03 antibody, MGC53388 antibody, PKM antibody, PK-S antibody, pk3 antibody, pkm2b antibody, zgc:92037 antibody, pyruvate kinase M1/2 antibody, pyruvate kinase, muscle antibody, Pyruvate kinase antibody, pyruvate kinase M1/2a antibody, pyruvate kinase PKM antibody, pyruvate kinase (PK) antibody, pyruvate kinase antibody, pyruvate kinase M1/2 S homeolog antibody, pyruvate kinase M1/2b antibody, PKM antibody, Pkm antibody, PyK antibody, pkma antibody, pkm antibody, LOC710798 antibody, pyk antibody, pk antibody, LOC100534543 antibody, pkm.S antibody, pkmb antibody

Background:

Background: Pyruvate kinase (PK) regulates the final rate-limiting step of glycolysis in the production of pyruvate and adenosine triphosphate (ATP). Alternate splicing of PKM pre-mRNA leads to PKM2 generation by the inclusion of exon 10 and the exclusion of exon 9, which is specific for PKM1. Besides its cytosolic roles in glycolysis, PKM2, which is upregulated by growth factor receptor activation 1, is phosphorylated at S37 by extracellular signal-regulated kinase (ERK) 2. This phosphorylation leads to the cis-trans isomerization of PKM2 by the peptidyl-prolineisomerase protein interacting with never in mitosis A 1 (PIN1), exposure of the nuclear localization signal (NLS) of PKM2, and subsequent binding of importin α 5 for nuclear translocation 2. In the nucleus, PKM2binds to phosphorylated Y333 of β-catenin, which is essential for β-catenin transactivation 3, and interacts with and phosphorylates histone H3 at T11, leading to H3-K9 acetylation and transcription of genes such as MYC and CCND14. c-Myc expression results in the upregulation of GLUT1, lactate dehydrogenase A (LDHA), and, in a positive feedback loop, PTB-dependent PKM2, which subsequently enhances the Warburg effect 2. Cyclin D1 expression, in turn, promotes G1-S phase transition 3, 4.1 Yang W, Xia Y, Cao Y et al. EGFR-induced and PKCepsilonmonoubiquitylation-dependent NF-kappaB activation upregulates PKM2 expression and promotes tumorigenesis. Molecular cell 2012; 48:771-784.2 Yang W, Zheng Y, Xia Y et al. ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect. Nature cell biology 2012; 14:1295-1304.3 Yang W, Xia Y, Ji H et al. Nuclear PKM2 regulates beta-catenin transactivation upon EGFR activation. Nature 2011; 480:118-122.4 Yang W, Xia Y, Hawke D et al. PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis. Cell 2012; 150:685-696.

Aliases: PKM; PK3; OIP3; PK2;

UniProt: P14618

Pathways: Warburg Effect