TERT antibody (pSer1125)

Catalog No. ABIN712046

Product Details anti-TERT Antibody

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Binding Specificity: pSer1125

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TERT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human TERT around the phosphorylation site of Ser1125

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for TERT

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Alternative Name: TERT (TERT Products)

Background:

Synonyms: p-TERTSer1125, TERTphospho S1125, EST2, hEST2, TCS1, Telomerase associated protein 2, Telomerase Catalytic Subunit, Telomere Reverse Transcriptase, TERT, TP2, TRT.

Background: Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Telomerase are large DNA-protein complexes with telomerase expression being the subject of recent research due to its link to cell immortalization. Recent evidence has shown that MYC upregulates the catalytic subunit of telomerase, TERT, and that TERT cooperates with HPV E7 in cell immortalization. Ever since the discovery that telomeres are short in cancer cells and telomerase is activated in immortal cells, telomerase has been associated with oncogenes. During the past year, major advances have been made in understanding the link between telomerase expression and cell immortality. Studies of yeast telomeres have revealed an unexpected role for the non-homologous end-joining machinery in telomere maintenance and have provided the first definitive evidence that telomeres play a critical role in meiosis. Identification of new telomere proteins has led to a better understanding of vertebrate telomere structure and function.

Gene ID: 7015

Pathways: Telomere Maintenance