SMAD4 antibody (AA 27-195)

Catalog No. ABIN7160238

Product Details anti-SMAD4 Antibody

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Binding Specificity: AA 27-195

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Mothers against decapentaplegic homolog 4 protein (27-195AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4 (SMAD4 Products)

Background:

Background: In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5\\\'-GTCT/AGAC-3\\\') within BMP response element (BMPRE) of cardiac activating regions. Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site, required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Aliases: (Small) Mothers Against Decapentaplegic antibody, Deleted in Pancreatic Carcinoma 4 antibody, Deleted in Pancreatic Carcinoma antibody, Deleted in pancreatic carcinoma locus 4 antibody, Deletion target in pancreatic carcinoma 4 antibody, DPC 4 antibody, DPC4 antibody, hSMAD4 antibody, JIP antibody, MAD homolog 4 antibody, MAD mothers against decapentaplegic Drosophila homolog 4 antibody, MAD mothers against decapentaplegic homolog 4 antibody, MADH 4 antibody, MADH4 antibody, Med antibody, Medea antibody, Mothers against decapentaplegic homolog 4 antibody, Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody, Mothers against DPP homolog 4 antibody, MYHRS antibody, OTTHUMP00000163548 antibody, SMA- and MAD-related protein 4 antibody, SMAD 4 antibody, SMAD family member 4 antibody, SMAD mothers against DPP homolog 4 antibody, SMAD4 antibody, SMAD4_HUMAN antibody

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy