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CD14 antibody (AA 20-345)

Name: CD14 antibody (AA 20-345)
Brand: antibodies-online
SKU: ABIN7160185
Price: 385 USD
Availability: InStock

CD14 Reactivity: Human FACS, WB, IHC, ELISA, IF Host: Mouse Monoclonal 14G1F3 unconjugated

Western Blot Positive WB detected in: Rabbit spleen tissue, Rabbit small intestine tissue All lanes: CD14 antibody at 1:2500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 55 kDa (CD14 antibody (AA 20-345))

Immunofluorescence staining of A549 cells with ABIN7160185 at 1:90, counter-stained with DAPI. (CD14 antibody (AA 20-345))

Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate All lanes: CD14 antibody at 1:1800 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 55 kDa (CD14 antibody (AA 20-345))

IHC image of ABIN7160185 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (CD14 antibody (AA 20-345))

9 images

Catalog No. ABIN7160185

Datasheet as PDF

Target See all CD14 Antibodies

CD14
Binding Specificity
9

5

5

5

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 20-345
Reactivity
221

37

30

25

22

18

16

15

14

13

13

10

9

9

8

8

8

5

2

2

2

1

1

1
Human
Host
191

52

16

3

1
Mouse
Compatible Secondaries
Clonality
209

54
Monoclonal
Conjugate
107

30

22

17

13

5

5

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
Application
196

77

55

49

45

43

20

13

13

11

7

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Cross-Reactivity

Human, Mouse, Rabbit

Purification

Protein A purified

Immunogen

Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)

Clone

14G1F3

Isotype

IgG1, IgG1 kappa

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CD14 Reactivity: Human IHC, ELISA Host: Mouse Monoclonal 1A3B6 unconjugated

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CD14 Reactivity: Human FACS, WB, IHC, ELISA Host: Mouse Monoclonal 1D1F8 unconjugated

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CD14 Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal LPSR-2385 unconjugated

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CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 unconjugated

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CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP, Func Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) (AA 201-300) antibody
CD14 Reactivity: Human, Mouse, Rat, Dog, Sheep, Rabbit WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD14 (CD14) antibody (Biotin)
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 Biotin

Application Notes

Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

CD14

Alternative Name

CD14 (CD14 Products)

Synonyms

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background

Background: Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187).
Aliases: Monocyte differentiation antigen CD14 (Myeloid cell-specific leucine-rich glycoprotein) (CD antigen CD14) [Cleaved into: Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form], CD14

UniProt

P08571

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades