BUB1 antibody (AA 256-577)

Catalog No. ABIN7160084

Product Details anti-BUB1 Antibody

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Binding Specificity: AA 256-577

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Mitotic checkpoint serine/threonine-protein kinase BUB1 protein (256-577AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: BUB1 (BUB1 Products)

Synonyms: bub1a antibody, bub1l antibody, hbub1 antibody, xbub1 antibody, BUB1A antibody, BUB1L antibody, hBUB1 antibody, AL022991 antibody, Bub1a antibody, C80208 antibody, D2Xrf87 antibody, BUB1 mitotic checkpoint serine/threonine kinase antibody, BUB1, mitotic checkpoint serine/threonine kinase antibody, BUB1 antibody, bub1 antibody, Bub1 antibody

Background:

Background: Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

Aliases: Bub1 antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody, BUB1 mitotic checkpoint serine/threonine kinase antibody, BUB1, S. cerevisiae, homolog of antibody, BUB1_HUMAN antibody, BUB1A antibody, BUB1L antibody, Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody, Budding uninhibited by benzimidazoles 1 homolog antibody, Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody, hBUB1 antibody, Homolog of mitotic checkpoint gene BUB1 antibody, Mitotic checkpoint gene BUB1 antibody, Mitotic checkpoint serine/threonine protein kinase BUB1 antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody, Mitotic spindle checkpoint kinase antibody, Putative serine/threonine protein kinase antibody

UniProt: O43683