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Nanog antibody (AA 1-305)

Name: Nanog antibody (AA 1-305)
Brand: antibodies-online
SKU: ABIN7155816
Price: 385 USD
Availability: InStock

NANOG Reactivity: Human WB, ELISA, IF, FACS, ICC Host: Mouse Monoclonal 8A1D11 unconjugated

Western Blot Positive WB detected in: Rat brain tissue, Mouse brain tissue All lanes: NANOG antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 46, 42 kDa (Nanog antibody (AA 1-305))

Overlay histogram showing Ntera-2 cells stained with ABIN7155816 (red line) at 1:250. (Nanog antibody (AA 1-305))

FACS

Western Blot Positive WB detected in: MCF-7 whole cell lysate, Ntera-2 whole cell lysate, A549 whole cell lysate All lanes: NANOG antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 46, 40 kDa (Nanog antibody (AA 1-305))

Immunocytochemistry analysis of ABIN7155816 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. (Nanog antibody (AA 1-305))

ICC

Immunocytochemistry analysis of ABIN7155816 diluted at 1:100 and staining in Ntera-2 cells performed on a Leica BondTM system. (Nanog antibody (AA 1-305))

ICC

Immunofluorescence staining of Hela cells with ABIN7155816 at 1:100, counter-stained with DAPI. (Nanog antibody (AA 1-305))

Immunofluorescence staining of Ntera-2 cells with ABIN7155816 at 1:100, counter-stained with DAPI. (Nanog antibody (AA 1-305))

Overlay histogram showing Hela cells stained with ABIN7155816 (red line) at 1:250. (Nanog antibody (AA 1-305))

FACS

Overlay histogram showing MCF-7 cells stained with ABIN7155816 (red line) at 1:250. (Nanog antibody (AA 1-305))

FACS

9 images

Catalog No. ABIN7155816

Datasheet as PDF

Target See all Nanog (NANOG) Antibodies

Nanog (NANOG) (Nanog Homeobox (NANOG))
Binding Specificity
16

16

12

10

8

7

7

6

6

5

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-305
Reactivity
137

58

22

4

4

4

3

3

2

2

1

1

1
Human
Host
127

15

3

1
Mouse
Compatible Secondaries
Clonality
122

24
Monoclonal
Conjugate
75

9

8

8

7

5

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
This Nanog antibody is un-conjugated
Application
108

60

43

42

26

26

21

18

17

15

8

7

5

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Cross-Reactivity

Human, Mouse, Rat

Purification

Protein G purified

Immunogen

Recombinant Human Homeobox protein NANOG protein (1-305AA)

Clone

8A1D11

Isotype

IgG1, IgG1 kappa

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anti-Nanog Homeobox (NANOG) antibody
NANOG Reactivity: Human WB, ELISA, IF, IHC, ICC, IHC (p), IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 101-200) antibody
NANOG Reactivity: Human, Mouse, Rat WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 71-170) antibody
NANOG Reactivity: Human, Mouse WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 15-49) antibody
NANOG Reactivity: Human WB, ELISA, IF, IHC, FACS Host: Rabbit Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 20-166) antibody
NANOG Reactivity: Human WB, ELISA, ICC Host: Mouse Monoclonal 1E6C4 unconjugated

anti-Nanog Homeobox (NANOG) (Internal Region) antibody
Verified NANOG Reactivity: Human WB, ELISA, IF, IHC Host: Goat Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2C11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3A12 unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-100) antibody
NANOG Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 267-292) antibody
NANOG Reactivity: Human WB, ELISA, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:50-1:200,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name

NANOG (NANOG Products)

Synonyms

2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody

Background

Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Aliases: Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog), NANOG

UniProt

Q9H9S0

Pathways

Stem Cell Maintenance