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GAPDH antibody (AA 3-335)

Name: GAPDH antibody (AA 3-335)
Brand: antibodies-online
SKU: ABIN7153644
Price: 264 USD
Availability: InStock

GAPDH Reactivity: Human WB, ELISA, IHC, IF, IP, FACS Host: Mouse Monoclonal 14C2F11 unconjugated

Western Blot Positive WB detected in: 15 μg hela whole cell lysate GAPDH antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 5 min (GAPDH antibody (AA 3-335))

IHC image of ABIN7153644 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. (GAPDH antibody (AA 3-335))

IHC

Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate All lanes: GAPDH antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 30s (GAPDH antibody (AA 3-335))

Western Blot Positive WB detected in: Hela whole cell lysate at 10 μg, 5 μg, 2. (GAPDH antibody (AA 3-335))

IHC image of ABIN7153644 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. (GAPDH antibody (AA 3-335))

IHC

5 images

Catalog No. ABIN7153644

Datasheet as PDF

Target See all GAPDH Antibodies

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Binding Specificity
34

20

13

13

7

6

5

5

4

4

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 3-335
Reactivity
210

144

127

63

46

39

25

25

23

19

19

19

17

15

14

14

10

9

8

6

4

4

4

4

4

4

4

3

3

2

2

2

2

1

1

1

1

1

1
Human
Host
162

82

16

11

4
Mouse
Compatible Secondaries
Clonality
185

88
Monoclonal
Conjugate
154

31

18

16

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This GAPDH antibody is un-conjugated
Application
219

100

72

65

38

34

29

26

16

15

15

15

7

6

5

4

3

3

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Cross-Reactivity

Human, Rabbit, Rat

Purification

Protein G purified

Immunogen

Recombinant Human GAPDH protein (3-335AA)

Clone

14C2F11

Isotype

IgG1, IgG1 kappa

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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH Reactivity: Human WB, IF, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH Reactivity: Human, Mouse, Rat WB, IF, IHC (p) Host: Mouse Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH Reactivity: Human, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 233-259), (C-Term) antibody
GAPDH Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB16543 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibody
GAPDH Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Recommended dilution: WB:1:5000-1:1600000, IHC:1:50-1:500, IF:1:50-1:200, IP:1 μL-2 μL, FC:1:100-1:300,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name

GAPDH (GAPDH Products)

Background

Background: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of 37 kDa that is consisdered as a cellular enzyme involved in glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. At the molecular level, sequential steps lead to nuclear translocation of GAPDH during cell death as follows: first, a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) that is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS), second, the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it, third, the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease.-deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
Aliases: GAPDH, G3PD, GAPD, MGC88685

UniProt

P04406