PHD1 antibody (AA 283-407)

Catalog No. ABIN7151444

Product Details anti-PHD1 Antibody

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Binding Specificity: AA 283-407

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHD1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Egl nine homolog 2 protein (283-407AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Alternative Name: EGLN2 (EGLN2 Products)

Synonyms: egln2 antibody, phd2 antibody, PHD1 antibody, 0610011A13Rik antibody, C85656 antibody, Hif-p4h-1 antibody, Ier4 antibody, Phd1 antibody, SM-20 antibody, HIF-PH1 antibody, HPH-1 antibody, HPH-3 antibody, PHD-1 antibody, EIT6 antibody, HIFPH1 antibody, egl-9 family hypoxia-inducible factor 1 antibody, egl-9 family hypoxia inducible factor 2 antibody, Egl nine homolog 2 antibody, egl-9 family hypoxia-inducible factor 2 L homeolog antibody, egl-9 family hypoxia-inducible factor 2 antibody, egln1 antibody, EGLN2 antibody, egln2 antibody, egln2.L antibody, Egln2 antibody

Background:

Background: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferencially recognized via a LXXLAP motif.

Aliases: DKFZp434E026 antibody, EGL nine (C.elegans) homolog 2 antibody, Egl nine homolog 2 (C. elegans) antibody, Egl nine homolog 2 antibody, EGLN 2 antibody, EGLN2 antibody, EGLN2_HUMAN antibody, EIT 6 antibody, EIT6 antibody, Estrogen-induced tag 6 antibody, HIF P4H 1 antibody, HIF PH1 antibody, HIF prolyl hydroxylase 1 antibody, HIF-PH1 antibody, HIF-prolyl hydroxylase 1 antibody, HIFPH 1 antibody, HIFPH1 antibody, HPH 3 antibody, HPH-1 antibody, HPH-3 antibody, HPH3 antibody, Hypoxia inducible factor prolyl hydroxylase 1 antibody, Hypoxia-inducible factor prolyl hydroxylase 1 antibody, P4H1 antibody, PHD 1 antibody, PhD1 antibody, prolyl hydroxylase domain containing protein 1 antibody, Prolyl hydroxylase domain-containing protein 1 antibody

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis