CHFR antibody (AA 141-422)

Catalog No. ABIN7151005

Product Details anti-CHFR Antibody

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Binding Specificity: AA 141-422

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHFR antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity: Human, Mouse

Purification: >95%, Protein G purified

Immunogen: Recombinant Human E3 ubiquitin-protein ligase CHFR protein (141-422AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for CHFR

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Alternative Name: CHFR (CHFR Products)

Synonyms: RNF116 antibody, RNF196 antibody, CHFR antibody, fc43f10 antibody, si:dkey-69h6.7 antibody, wu:fc43f10 antibody, rnf116 antibody, rnf196 antibody, 5730484M20Rik antibody, C230082M18 antibody, checkpoint with forkhead and ring finger domains antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog antibody, CHFR antibody, chfr antibody, Chfr antibody, chfr.L antibody

Background:

Background: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the \\\'Lys-48\\\'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of \\\'Lys-63\\\'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at \\\'Lys-63\\\' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Aliases: Checkpoint with forkhead and ring finger domains antibody, Checkpoint with forkhead and RING finger domains protein antibody, Chfr antibody, CHFR_HUMAN antibody, E3 ubiquitin protein ligase CHFR antibody, E3 ubiquitin-protein ligase CHFR antibody, EC 6.3.2. antibody, FLJ10796 antibody, FLJ33629 antibody, RING finger protein 196 antibody, RNF 116 antibody, RNF 196 antibody, RNF116 antibody, RNF196 antibody, Ubiquitin ligase protein antibody

UniProt: Q96EP1