TOP1MT antibody (AA 270-400) (FITC)
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- Target See all TOP1MT Antibodies
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
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Binding Specificity
- AA 270-400
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This TOP1MT antibody is conjugated to FITC
- Application
- Please inquire
- Cross-Reactivity
- Human
- Purification
- >95%, Protein G purified
- Immunogen
- Recombinant Human DNA topoisomerase I, mitochondrial protein (270-400AA)
- Isotype
- IgG
- Top Product
- Discover our top product TOP1MT Primary Antibody
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4 - Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- Target
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
- Alternative Name
- TOP1MT (TOP1MT Products)
- Synonyms
- 2900052H09Rik antibody, TOP1 antibody, topoisomerase (DNA) I, mitochondrial antibody, DNA topoisomerase 1, mitochondrial antibody, DNA topoisomerase I mitochondrial antibody, TOP1MT antibody, Top1mt antibody
- Background
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Background: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3\'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5\'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5\'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Aliases: TOP1MT antibody, DNA topoisomerase I antibody, mitochondrial antibody, TOP1mt antibody, EC 5.6.2.1 antibody
- UniProt
- Q969P6
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