TOP1MT antibody (AA 270-400)

Catalog No. ABIN7150351

Product Details anti-TOP1MT Antibody

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Binding Specificity: AA 270-400

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP1MT antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human DNA topoisomerase I, mitochondrial protein (270-400AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for TOP1MT

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Alternative Name: TOP1MT (TOP1MT Products)

Synonyms: 2900052H09Rik antibody, TOP1 antibody, topoisomerase (DNA) I, mitochondrial antibody, DNA topoisomerase 1, mitochondrial antibody, DNA topoisomerase I mitochondrial antibody, TOP1MT antibody, Top1mt antibody

Background:

Background: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3\\\'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5\\\'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5\\\'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Aliases: TOP1MT antibody, DNA topoisomerase I antibody, mitochondrial antibody, TOP1mt antibody, EC 5.6.2.1 antibody

UniProt: Q969P6