NDEL1 antibody (pSer231)

Catalog No. ABIN710801

Product Details anti-NDEL1 Antibody

Target: NDEL1 (NudE Nuclear Distribution E Homolog (A. Nidulans)-Like 1 (NDEL1))

Binding Specificity: pSer231

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDEL1 antibody is un-conjugated

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human NDEL1 around the phosphorylation site of Ser231

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NDEL1

Target: NDEL1 (NudE Nuclear Distribution E Homolog (A. Nidulans)-Like 1 (NDEL1))

Alternative Name: NDEL1 (NDEL1 Products)

Synonyms: EOPA antibody, MITAP1 antibody, NDE1L1 antibody, NDE2 antibody, NUDEL antibody, NUDE2 antibody, Nudel antibody, 2600006O07Rik antibody, mNudel antibody, wu:fi23c04 antibody, wu:fi23e06 antibody, eopa antibody, mitap1 antibody, ndel1 antibody, ndel1-a antibody, ndel1-b antibody, nudel antibody, nudE neurodevelopment protein 1 like 1 antibody, nudE neurodevelopment protein 1-like 1 antibody, nudE neurodevelopment protein 1-like 1b antibody, nudE neurodevelopment protein 1 like 1 L homeolog antibody, NDEL1 antibody, Ndel1 antibody, ndel1b antibody, ndel1.L antibody

Background:

Synonyms: NDEL1phospho S231, Nudelphospho S231, A. nidulans, DKFZp451M0318, ENDOOLIGOPEPTIDASE A, EOPA, MITAP 1, MITAP1, Mitosin associated protein 1, Mitosin associated protein MITAP1, Mitosin-associated protein 1, Ndel 1, Ndel1, NDEL1_HUMAN, Nuclear distribution gene E like homolog 1, Nuclear distribution protein nudE like 1, Nuclear distribution protein nudE-like 1, NUDE like protein, NudE nuclear distribution gene E homolog like 1 A. nidulans, NudE nuclear distribution gene E homolog like 1, NUDEL, Protein Nudel.

Background: Nudel is important for normal cortical development. It is invovled in microtubule organization, nuclear translocation, and neuronal positioning in concert with various other factors (including Lis1, Pafah1b1, Pahfah1b2, dynein, dynorphin A and cdk5). Western blot analysis of mouse tissues shows abundant expression of Nudel in brain and testis, and much lower expression in heart, liver, kidney, and skeletal muscle. In fractionated rat brain, Nudel and Lis1 are both found in fractions enriched for postsynaptic density proteins. Immunostaining of embryonic day 18 mouse brain sections revealed staining of migrating neurons and thalamocortical axons of the intermediate zone of the developing cerebral cortex, as well as several other developing brain regions. The deduced protein contains 345 amino acids and has a calculated molecular mass of 38.4 kDa. It has a coiled coil motif (residues 19 to 201), followed by several potential phosphorylation sites for casein kinase II, protein kinase C or CDK5. Nudel shares about 50 % identity with mouse and human NUDE proteins.

Gene ID: 81565

Pathways: Regulation of Cell Size