POLD1 antibody (Catalytic Subunit) (Biotin)
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- Target See all POLD1 Antibodies
- POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))
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Binding Specificity
- AA 1-123, Catalytic Subunit
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLD1 antibody is conjugated to Biotin
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Application
- ELISA
- Cross-Reactivity
- Human
- Purification
- >95%, Protein G purified
- Immunogen
- Recombinant Human DNA polymerase delta catalytic subunit protein (1-123AA)
- Isotype
- IgG
- Top Product
- Discover our top product POLD1 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4 - Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- Target
- POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))
- Alternative Name
- POLD1 (POLD1 Products)
- Synonyms
- CDC2 antibody, CRCS10 antibody, POLD antibody, 5949 antibody, CAA61369 antibody, CG5949 antibody, DNA Pol Delta antibody, Dm Poldelta antibody, Dmel\\CG5949 antibody, Poldelta antibody, dnapol delta antibody, l(3)72Ac antibody, cdc2 antibody, pold antibody, 125kDa antibody, Pol delta antibody, fla antibody, im:7139126 antibody, zgc:152688 antibody, DNA polymerase delta 1, catalytic subunit antibody, DNA-polymerase-delta antibody, polymerase (DNA directed), delta 1, catalytic subunit antibody, DNA polymerase delta catalytic subunit antibody, DNA polymerase delta antibody, POLD1 antibody, DNApol-delta antibody, pold1 antibody, LOC100456571 antibody, Pold1 antibody
- Background
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Background: As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3\'- to 5\'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5\'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196).
Aliases: Polymerase (DNA directed) delta 1 catalytic subunit antibody, CDC2 antibody, CDC2 homolog antibody, CRCS10 antibody, DNA directed DNA polymerase delta 1 antibody, DNA directed polymerase delta 1 antibody, DNA pol delta 1 antibody, DNA polymerase delta catalytic subunit antibody, DNA polymerase subunit delta p125 antibody, DPOD1_HUMAN antibody, MDPL antibody, POLD antibody, POLD 1 antibody, POLD1 antibody, Polymerase (DNA directed) delta 1 catalytic subunit 125 kDa antibody, Polymerase (DNA) delta 1 catalytic subunit antibody, Polymerase DNA directed delta 1 catalytic subunit 125kD antibody, polymerase, DNA, delta antibody
- UniProt
- P28340
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA
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