Cullin 7 antibody (AA 312-450) (HRP)

Catalog No. ABIN7148763

Product Details anti-Cullin 7 Antibody (HRP)

Target: Cullin 7 (CUL7)

Binding Specificity: AA 312-450

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 7 antibody is conjugated to HRP

Application: ELISA

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Cullin-7 protein (312-450AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Cullin 7

Target: Cullin 7 (CUL7)

Alternative Name: CUL7 (CUL7 Products)

Synonyms: cullin-7 antibody, CUL7 antibody, Cullin-7 antibody, KIAA0076 antibody, dJ20C7.5 antibody, 2510004L20Rik antibody, AA409809 antibody, C230011P08Rik antibody, p185 antibody, p193 antibody, cullin 7 antibody, CUL7 antibody, Cul7 antibody

Background:

Background: Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediates the ubiquitination of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed:24793695). Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5 (PubMed:24793696). Core component of a Cul7-RING ubiquitin-protein ligase with FBXW8, which mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1 (PubMed:21572988, PubMed:24362026). Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain (PubMed:21572988). Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2) (PubMed:18498745). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradatation, thereby affecting cell proliferation and differentiation (PubMed:24362026). Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development (PubMed:20139075). Does not promote polyubiquitination and proteasomal degradation of p53/TP53 (PubMed:16547496, PubMed:17332328). While the Cul7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING(FBXW8) complex may be have additional functions.

Aliases: CUL-7 antibody, CUL7 antibody, CUL7_HUMAN antibody, Cullin 7 antibody, Cullin-7 antibody, dJ20C7.5 antibody, KIAA0076 antibody

UniProt: Q14999

Pathways: ER-Nucleus Signaling