CH25H antibody (AA 142-272)

Catalog No. ABIN7147600

Product Details anti-CH25H Antibody

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Binding Specificity: AA 142-272

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CH25H antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Cholesterol 25-hydroxylase protein (142-272AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for CH25H

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Alternative Name: CH25H (CH25H Products)

Synonyms: CH25H antibody, zgc:101688 antibody, MGC143045 antibody, C25H antibody, AI462618 antibody, m25OH antibody, cholesterol 25-hydroxylase antibody, cholesterol 25-hydroxylase L homeolog antibody, CH25H antibody, ch25h antibody, Ch25h antibody, ch25h.L antibody

Background:

Background: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

Aliases: CH25H antibody, Cholesterol 25-hydroxylase antibody, EC 1.14.99.38 antibody, Cholesterol 25-monooxygenase antibody, h25OH antibody

UniProt: O95992