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MCAM antibody (AA 50-646)

Name: MCAM antibody (AA 50-646)
Brand: antibodies-online
SKU: ABIN7147325
Price: 385 USD
Availability: InStock

MCAM Reactivity: Human FACS, WB, IF, ELISA Host: Mouse Monoclonal 4D10C7 unconjugated

Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CD146 antibody at 1:1250 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 72, 58 kDa Observed band size: 120 kDa (MCAM antibody (AA 50-646))

Overlay histogram showing MCF-7 cells stained with ABIN7147325 (red line) at 1:400. (MCAM antibody (AA 50-646))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate All lanes: CD146 antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 72, 58 kDa Observed band size: 120 kDa (MCAM antibody (AA 50-646))

Immunofluorescence staining of A375 cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM antibody (AA 50-646))

Immunofluorescence staining of Hela cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM antibody (AA 50-646))

Immunofluorescence staining of MCF-7 cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM antibody (AA 50-646))

Immunofluorescence staining of THP-1 cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM antibody (AA 50-646))

7 images

Catalog No. ABIN7147325

Datasheet as PDF

Target See all MCAM Antibodies

MCAM (Melanoma Cell Adhesion Molecule (MCAM))
Binding Specificity
16

13

11

5

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 50-646
Reactivity
124

47

46

14

14

9

8

7

6

2
Human
Host
96

52

1

1

1
Mouse
Compatible Secondaries
Clonality
89

62
Monoclonal
Conjugate
70

14

12

9

4

3

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This MCAM antibody is un-conjugated
Application
84

68

65

42

18

17

14

10

10

5

4

4

3

3

2

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), ELISA

Cross-Reactivity

Human

Purification

Protein G purified

Immunogen

Recombinant Human Cell surface glycoprotein MUC18 protein (50-646AA)

Clone

4D10C7

Isotype

IgG2a

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Application Notes

Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name

CD146 (MCAM Products)

Synonyms

CD146 antibody, MUC18 antibody, Muc18 antibody, 1-gicerin antibody, AV025631 antibody, CD149 antibody, s-endo antibody, s-gicerin antibody, cd146 antibody, gicerin antibody, id:ibd3512 antibody, mcam antibody, MCAM antibody, melanoma cell adhesion molecule antibody, melanoma cell adhesion molecule b antibody, MCAM antibody, Mcam antibody, mcamb antibody

Background

Background: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Aliases: Cell surface glycoprotein MUC18 (Cell surface glycoprotein P1H12) (Melanoma cell adhesion molecule) (Melanoma-associated antigen A32) (Melanoma-associated antigen MUC18) (S-endo 1 endothelial-associated antigen) (CD antigen CD146), MCAM, MUC18

UniProt

P43121