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CD44 antibody (AA 21-220)

Name: CD44 antibody (AA 21-220)
Brand: antibodies-online
SKU: ABIN7147160
Price: 385 USD
Availability: InStock

CD44 Reactivity: Human FACS, WB, IHC, ELISA, IF Host: Mouse Monoclonal 6G2D2 unconjugated

Western Blot Positive WB detected in: Hela whole cell lysate, HUVEC whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate All lanes: CD44 antibody at 1:1500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54,47, 40, 44, 33, 74, 76, 38, 16 kDa Observed band size: 95 kDa (CD44 antibody (AA 21-220))

Overlay histogram showing Hela cells stained with ABIN7147160 (red line) at 1:200. (CD44 antibody (AA 21-220))

FACS

Western Blot Positive WB detected in: A549 whole cell lysate All lanes: CD44 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54,47, 40, 44, 33, 74, 76, 38, 16 kDa Observed band size: 95 kDa (CD44 antibody (AA 21-220))

IHC image of ABIN7147160 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (CD44 antibody (AA 21-220))

IHC

IHC image of ABIN7147160 diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. (CD44 antibody (AA 21-220))

IHC

IHC image of ABIN7147160 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. (CD44 antibody (AA 21-220))

IHC

Immunofluorescence staining of Hela cells with ABIN7147160 at 1:100, counter-stained with DAPI. (CD44 antibody (AA 21-220))

Immunofluorescence staining of MCF-7 cells with ABIN7147160 at 1:100, counter-stained with DAPI. (CD44 antibody (AA 21-220))

8 images

Catalog No. ABIN7147160

Datasheet as PDF

Target See all CD44 Antibodies

CD44
Binding Specificity
16

16

16

10

8

5

4

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 21-220
Reactivity
282

136

73

32

25

23

15

13

10

9

8

7

7

5

5

4

2

2

1

1

1

1

1
Human
Host
175

117

73

2

1
Mouse
Compatible Secondaries
Clonality
261

105

2
Monoclonal
Conjugate
171

45

31

22

17

7

6

5

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This CD44 antibody is un-conjugated
Application
275

149

91

73

71

68

57

41

40

30

27

15

7

6

6

5

5

2

2

2

2

2

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Cross-Reactivity

Human

Purification

Protein G purified

Immunogen

Recombinant Human CD44 antigen protein (21-220AA)

Clone

6G2D2

Isotype

IgG1

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CD44 Reactivity: Human, Mouse, Rat WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

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Application Notes

Recommended dilution: WB:1:1000-1:40000, IHC:1:50-1:200, IF:1:50-1:200,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

CD44

Alternative Name

CD44 (CD44 Products)

Background

Background: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Receptor for LGALS9, the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity).
Aliases: CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, CD44, LHR, MDU2, MDU3, MIC4

UniProt

P16070

Pathways

Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling