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CD19 antibody (AA 20-291)

Name: CD19 antibody (AA 20-291)
Brand: antibodies-online
SKU: ABIN7147133
Price: 462 USD
Availability: InStock

CD19 Reactivity: Human FACS, WB, IHC, IF, ELISA Host: Mouse Monoclonal 8G11B7 unconjugated

Overlay histogram showing Raji cells stained with ABIN7147133 (red line) at 1:100. (CD19 antibody (AA 20-291))

FACS

Western Blot Positive WB detected in: Raji whole cell lysate All lanes: CD19 antibody at 1:2000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 61 kDa Observed band size: 95 kDa (CD19 antibody (AA 20-291))

Western Blot Positive WB detected in: Raji whole cell lysate All lanes: CD19 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 61 kDa Observed band size: 95 kDa (CD19 antibody (AA 20-291))

IHC image of ABIN7147133 diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. (CD19 antibody (AA 20-291))

IHC

Immunofluorescence staining of Hela cells with ABIN7147133 at 1:100, counter-stained with DAPI. (CD19 antibody (AA 20-291))

IHC image of ABIN7147133 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (CD19 antibody (AA 20-291))

IHC

Immunofluorescence staining of Raji cells with ABIN7147133 at 1:100, counter-stained with DAPI. (CD19 antibody (AA 20-291))

7 images

Catalog No. ABIN7147133

Datasheet as PDF

Target See all CD19 Antibodies

CD19 (CD19 Molecule (CD19))
Binding Specificity
21

16

16

14

10

7

4

4

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 20-291
Reactivity
274

153

39

28

19

10

6

5

3

3

2

2

2

2

2

2

2

2
Human
Host
170

124

48

4
Mouse
Compatible Secondaries
Clonality
228

113

3
Monoclonal
Conjugate
115

24

22

22

17

8

8

8

8

6

5

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This CD19 antibody is un-conjugated
Application
258

108

65

63

53

48

22

21

14

14

10

9

5

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Cross-Reactivity

Human

Purification

Protein A purified

Immunogen

Recombinant Human CD19 protein (20-291AA)

Clone

8G11B7

Isotype

IgG1, IgG1 kappa

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Application Notes

Recommended dilution: WB:1:2000-1:80000, IHC:1:50-1:200, IF:1:50-1:200,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

CD19 (CD19 Molecule (CD19))

Alternative Name

CD19 (CD19 Products)

Background

Background: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Aliases: B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19, CD19

UniProt

P15391

Pathways

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway