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CD19 antibody (AA 20-291)

Name: CD19 antibody (AA 20-291)
Brand: antibodies-online
SKU: ABIN7147133
Price: 385 USD
Availability: InStock

CD19 Reactivity: Human FACS, WB, IHC, ELISA, IF Host: Mouse Monoclonal 8G11B7 unconjugated

Overlay histogram showing Raji cells stained with ABIN7147133 (red line) at 1:100. (CD19 antibody (AA 20-291))

FACS

Western Blot Positive WB detected in: Raji whole cell lysate All lanes: CD19 antibody at 1:2000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 61 kDa Observed band size: 95 kDa (CD19 antibody (AA 20-291))

Western Blot Positive WB detected in: Raji whole cell lysate All lanes: CD19 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 61 kDa Observed band size: 95 kDa (CD19 antibody (AA 20-291))

IHC image of ABIN7147133 diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. (CD19 antibody (AA 20-291))

IHC

Immunofluorescence staining of Hela cells with ABIN7147133 at 1:100, counter-stained with DAPI. (CD19 antibody (AA 20-291))

IHC image of ABIN7147133 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (CD19 antibody (AA 20-291))

IHC

Immunofluorescence staining of Raji cells with ABIN7147133 at 1:100, counter-stained with DAPI. (CD19 antibody (AA 20-291))

7 images

Catalog No. ABIN7147133

Datasheet as PDF

Target See all CD19 Antibodies

CD19 (CD19 Molecule (CD19))
Binding Specificity
30

19

16

16

10

7

5

4

4

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 20-291
Reactivity
287

158

48

31

19

10

7

5

4

3

2

2

2

2

2

2

2

2
Human
Host
168

138

43

4
Mouse
Compatible Secondaries
Clonality
220

129

2
Monoclonal
Conjugate
114

24

23

21

18

8

8

7

7

7

6

5

5

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This CD19 antibody is un-conjugated
Application
250

116

70

61

50

44

22

21

14

14

10

10

5

4

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Cross-Reactivity

Human

Purification

Protein A purified

Immunogen

Recombinant Human CD19 protein (20-291AA)

Clone

8G11B7

Isotype

IgG1, IgG1 kappa

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Application Notes

Recommended dilution: WB:1:2000-1:80000, IHC:1:50-1:200, IF:1:50-1:200,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

CD19 (CD19 Molecule (CD19))

Alternative Name

CD19 (CD19 Products)

Synonyms

B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody

Background

Background: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Aliases: B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19, CD19

UniProt

P15391

Pathways

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway