BRD4 antibody (AA 150-200)

Catalog No. ABIN7145812

Product Details anti-BRD4 Antibody

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Binding Specificity: AA 150-200

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD4 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Bromodomain-containing protein 4 protein (150-200AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IF:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for BRD4

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4 (BRD4 Products)

Synonyms: brd4-b antibody, BRD4 antibody, fb18g03 antibody, wu:fb18g03 antibody, CAP antibody, HUNK1 antibody, HUNKI antibody, MCAP antibody, Brd5 antibody, WI-11513 antibody, bromodomain containing 4 L homeolog antibody, bromodomain containing 4 antibody, bromodomain-containing protein 4 antibody, brd4.L antibody, BRD4 antibody, brd4 antibody, LOC100409351 antibody, Brd4 antibody

Background:

Background: Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of \\\'Ser-2\\\' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of \\\'Ser-2\\\' of the C-terminal domain (CTD) of RNA polymerase II, these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.

Aliases: Brd4 antibody, BRD4-NUT FUSION antibody, BRD4-NUT fusion oncoprotein antibody, BRD4_HUMAN antibody, Bromodomain containing 4 antibody, bromodomain containing protein 4 antibody, Bromodomain-containing protein 4 antibody, CAP antibody, chromosome associated protein antibody, HUNK1 antibody, HUNKI antibody, MCAP antibody, Mitotic chromosome-associated protein antibody, Protein HUNK1 antibody

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome