MOCS3 antibody (AA 279-404) (HRP)

Catalog No. ABIN7143367

Product Details anti-MOCS3 Antibody (HRP)

Target: MOCS3 (Molybdenum Cofactor Synthesis 3 (MOCS3))

Binding Specificity: AA 279-404

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MOCS3 antibody is conjugated to HRP

Application: ELISA

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Adenylyltransferase and sulfurtransferase MOCS3 protein (279-404aa)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MOCS3

Target: MOCS3 (Molybdenum Cofactor Synthesis 3 (MOCS3))

Alternative Name: MOCS3 (MOCS3 Products)

Synonyms: MOCS3 antibody, DDBDRAFT_0189692 antibody, DDBDRAFT_0267172 antibody, DDB_0189692 antibody, DDB_0267172 antibody, UBA4 antibody, 1700020H17Rik antibody, Uba4 antibody, zgc:55696 antibody, uba4 antibody, molybdenum cofactor synthesis 3 antibody, molybdenum cofactor synthesis 3 L homeolog antibody, MOCS3 antibody, mocs3 antibody, Mocs3 antibody, mocs3.L antibody

Background:

Background: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor, NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

Aliases: Adenylyltransferase and sulfurtransferase MOCS3 (Molybdenum cofactor synthesis protein 3) (Molybdopterin synthase sulfurylase) (MPT synthase sulfurylase) [Includes: Molybdopterin-synthase adenylyltransferase (EC 2.7.7.80) (Adenylyltransferase MOCS3) (Sulfur carrier protein MOCS2A adenylyltransferase), Molybdopterin-synthase sulfurtransferase (EC 2.8.1.11) (Sulfur carrier protein MOCS2A sulfurtransferase) (Sulfurtransferase MOCS3)], MOCS3, UBA4

UniProt: O95396