BRK1 antibody (AA 31-75)

Catalog No. ABIN710036

Product Details anti-BRK1 Antibody

Target: BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))

Binding Specificity: AA 31-75

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRK1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human hHBrk1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for BRK1

Target: BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))

Alternative Name: hHBrk1 (BRK1 Products)

Synonyms: brk1 antibody, C22H3orf10 antibody, brick1 antibody, c3orf10 antibody, C3orf10 antibody, MDS027 antibody, hHBrk1 antibody, 6720456B07Rik antibody, AW011779 antibody, ATBRK1 antibody, BRICK1 antibody, HSPC300 antibody, T9I22.8 antibody, T9I22_8 antibody, BRICK1, SCAR/WAVE actin-nucleating complex subunit antibody, BRICK1, SCAR/WAVE actin nucleating complex subunit antibody, brick 1 antibody, BRICK1, SCAR/WAVE actin-nucleating complex subunit S homeolog antibody, BRICK1 antibody, brk1 antibody, BRK1 antibody, Brk1 antibody, brk1.S antibody

Background:

Synonyms: Brk1-like, C3orf10 chromosome 3 open reading frame 10, C3ORF10 GENE, Chromosome 3 open reading frame 10, hHBrk1, HSPC300, MDS027, Probable protein BRICK1, BRK1_HUMAN.

Background: HSPC300 (hematopoietic stem cell protein 300) is also known as probable protein BRICK1 or C3orf10 (chromosome 3 open reading frame 10) and is a 75 amino acid protein that is expressed as two isoforms and localizes to both the cytoplasm and the cytoskeleton. HSPC300 is thought to regulate cytoskeletal organization and Actin polymerization. Free HSPC300 exists as homotrimers prior to its incorporation into the WAVE complex. The WAVE complex includes five proteins, one of which is HSPC300, that regulate the ARC (Arp2/3 complex) which is responsible for Actin nucleation and is Rac 1-dependent. Because HSPC300 is a highly conserved subunit of the WAVE complex across many species, it is thought to have the same or similar functions in many different organisms. In Drosophila, the WAVE/ARC pathway may affect the development of the nervous system. HSPC300 is thought to localize to axons of the central nervous system of Drosophila embryos and thus may also be involved in axonogenesis. In addition, HSPC300 is thought to be necessary for synaptic morphogenesis by motoneurons. In mice, the knockout of the WAVE complex leads to learning and memory defects, and it is therefore hypothesized that HSPC300 may also be involved in cognitive functions. Genetic depletion of HSPC300 results in cytoskeletal abnormalities and prevents cytokinesis of cells, suggesting that decreased levels of HSPC300 may be associated with tumor suppression.

Gene ID: 55845

Pathways: RTK Signaling