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GFP antibody

Name: GFP antibody
Brand: antibodies-online
SKU: ABIN7141875
Price: 220 USD
Availability: InStock

GFP Reactivity: Aequorea victoria WB, ELISA, IF, IP, FACS Host: Mouse Monoclonal 6C11C11 unconjugated

Western Blot Positive WB detected in: 50 ng recombinant protein All lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 KDa Exposure time:5 min (GFP antibody)

Immunofluorescence staining of 293F cells transfected with GFP with ABIN7141875 at 1:100, counter-stained with DAPI. (GFP antibody)

Western Blot Positive WB detected in: 1,2,3 and 4 are Recombinant proteins with GFP tag for 50 ng, 5, 293F whole cell lysate transfected with GFP for 5 μg All lanes GFP antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively Observed band size: 32,32,50,32,32 KDa Exposure time:1 min (GFP antibody)

Overlay histogram showing 293F cells transfected with GFP stained with ABIN7141875 (red line) at 1:200. (GFP antibody)

FACS

Western Blot Positive WB detected in: Recombinant protein at 25 ng, 12. (GFP antibody)

Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with ABIN7141875 at 1:200. (GFP antibody)

FACS

Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP Lane 1: Mouse control IgG2b instead of ABIN7141875 in 293F whole cell lysate transfected with GFP Lane 2: ABIN7141875 (4 μg) + 293F whole cell lysate transfected with GFP (500 μg) Lane 3: 293F whole cell lysate transfected with GFP (5 μg) For western blotting, the blot was detected with ABIN7141875 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000 (GFP antibody)

7 images

Catalog No. ABIN7141875

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
176

15

11

9
Aequorea victoria
Host
64

62

35

14

9

5

2

1
Mouse
Compatible Secondaries
Clonality
115

72
Monoclonal
Conjugate
83

12

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
Application
149

74

45

33

27

25

24

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Purification

Protein G purified

Immunogen

Recombinant GFP Protein

Clone

6C11C11

Isotype

IgG2b

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Application Notes

Recommended dilution: WB:1:50000-1:6400000, IF:1:50-1:200, FC:1:100-1:300, IP:1 μL-2 μL,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Background

Background: The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65-Tyr66-Gly67 that lead to chromophore formation. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has always been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.
Aliases: CFP, eGFP, eYFP, GFP, GFP tag, YFP