Cortactin antibody (pTyr466)

Catalog No. ABIN7139010

Product Details anti-Cortactin Antibody

Target: Cortactin (CTTN)

Binding Specificity: pTyr466

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Human, Mouse

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y(p)-E-T) derived from Human CORTACTIN.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: CTTN (CTTN Products)

Synonyms: EMS1 antibody, 1110020L01Rik antibody, Ems1 antibody, Cttnb antibody, CG3637 antibody, Dmel\CG3637 antibody, cortactin antibody, cttn antibody, CTTN1 antibody, P85.25 antibody, Cttn antibody, ems1 antibody, CTTN antibody, cortactin antibody, CG3637 gene product from transcript CG3637-RD antibody, cortactin S homeolog antibody, cortactin L homeolog antibody, src substrate cortactin antibody, Src substrate cortactin antibody, CTTN antibody, Cttn antibody, Cortactin antibody, cttn.S antibody, cttn.L antibody, cttn antibody, CpipJ_CPIJ006351 antibody, Bm1_57220 antibody, LOC100533277 antibody

Background:

Background:

Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

Luo C, et al. (2006) J Biol Chem , 281(40):30081-30093
Head JA, et al. (2003) Mol Biol Cell ,14(8):3216-3229
Li Y, et al. (2000) J Biol Chem , 275(47): 37187-37193
Kinley AW, et al. (2003) Curr Biol , 13(5): 384-393

Aliases: Amplaxin antibody, CTTN antibody, EMS 1 antibody, EMS1 antibody, FLJ34459 antibody, Mammary tumor and squamous cell carcinoma associated antibody, Oncogene EMS1 antibody, p80/85 src substrate antibody, Src substrate cortactin antibody, SRC8_HUMAN antibody

UniProt: Q14247

Pathways: MAPK Signaling