SHC1 antibody (pTyr427)
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- Target See all SHC1 Antibodies
- SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
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Binding Specificity
- pTyr427
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SHC1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
- Immunogen
- Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N derived from Human Shc1.
- Isotype
- IgG
- Top Product
- Discover our top product SHC1 Primary Antibody
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- Application Notes
- WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- Target
- SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
- Alternative Name
- SHC1 (SHC1 Products)
- Synonyms
- SHC1 antibody, SHC antibody, SHCA antibody, P66shc antibody, si:dkey-246i14.4 antibody, wu:fc10h03 antibody, shc antibody, p66shc antibody, Shc antibody, ShcA antibody, p66 antibody, SHC adaptor protein 1 antibody, Shc1p antibody, squalene--hopene cyclase antibody, SHC (Src homology 2 domain containing) transforming protein 1 antibody, SHC (Src homology 2 domain containing) transforming protein 1 L homeolog antibody, src homology 2 domain-containing transforming protein C1 antibody, SHC1 antibody, shc1 antibody, Shc1 antibody, shc1.L antibody
- Background
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Background: Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span
Trampont P, et al. (2006) Mol Cell Biol, 26(23): 9035-9044.
Patrussi L, et al. (2005) Oncogene, 24(13): 2218-2228
van der Geer P, et al. (1996) Curr Biol , 6(11): 1435-1444Aliases: FLJ26504 antibody, p66 antibody, p66SHC antibody, SH2 domain protein C1 antibody, SHC (Src homology 2 domain containing) transforming protein 1 antibody, SHC 1 antibody, SHC A antibody, SHC adaptor protein 1 antibody, Shc antibody, SHC transforming protein 1 antibody, SHC transforming protein antibody, SHC-transforming protein 1 antibody, SHC-transforming protein 3 antibody, SHC-transforming protein A antibody, SHC1 antibody, SHC1_HUMAN antibody, SHCA antibody, Src homology 2 domain-containing-transforming protein C1 antibody
- UniProt
- P29353
- Pathways
- RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2
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