SYK antibody (pTyr323)

Catalog No. ABIN7138980

Product Details anti-SYK Antibody

Target: SYK (Spleen tyrosine Kinase (SYK))

Binding Specificity: pTyr323

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Cross-Reactivity: Human

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 323 (N-P-Y(p)-E- P) derived from Human syk.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: SYK (SYK Products)

Synonyms: p72-Syk antibody, Sykb antibody, p72syk antibody, spleen associated tyrosine kinase antibody, spleen tyrosine kinase antibody, syk antibody, SYK antibody, Syk antibody

Background:

Background: Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion

Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.
Turner, M. et al. (2000) Immunol. Today 21, 148-154.
Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.
Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.

Aliases: EC 2.7.10.2 antibody, kinase Syk antibody, KSYK antibody, KSYK_HUMAN antibody, p72-Syk antibody, p72syk antibody, Spleen tyrosine kinase antibody, Syk antibody, Tyrosine protein kinase SYK antibody, Tyrosine-protein kinase SYK antibody

UniProt: P43405

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling