MYL9 antibody (pTyr118)

Catalog No. ABIN7138917

Product Details anti-MYL9 Antibody

Target: MYL9 (Myosin Regulatory Light Polypeptide 9 (MYL9))

Binding Specificity: pTyr118

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Cross-Reactivity: Human

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 118 (A-D-Y(p)-V-R) derived from Human MLC2.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MYL9

Target: MYL9 (Myosin Regulatory Light Polypeptide 9 (MYL9))

Alternative Name: MYL9 (MYL9 Products)

Synonyms: LC20 antibody, MLC-2C antibody, MLC2 antibody, MRLC1 antibody, MYRL2 antibody, AI327049 antibody, MLC20 antibody, Mylc2c antibody, RLC-C antibody, myosin antibody, regulatory antibody, MRCL3 antibody, MRLC2 antibody, MYL9 antibody, zgc:103467 antibody, myl9 antibody, zgc:77916 antibody, myosin light chain 9 antibody, myosin, light polypeptide 9, regulatory antibody, myosin light chain 9 L homeolog antibody, myosin, light chain 9, regulatory antibody, myosin, light chain 12A, regulatory, non-sarcomeric antibody, myosin, light chain 9a, regulatory antibody, myosin, light chain 9b, regulatory antibody, MYL9 antibody, Myl9 antibody, myl9.L antibody, MYL12A antibody, myl9a antibody, myl9b antibody

Background:

Background:  Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. 1) Xia, Y. et al. c-Jun downregulation by HDAC3-dependent transcriptional repression promotes osmotic stress-induced cell apoptosis. Mol. Cell 25, 219–232 (2007).2)Vander Heiden, M. G. et al. Evidence for an alternative glycolytic pathway in rapidly proliferating cells. Science 329, 1492–1499 (2010).3) Fang, D. et al. Phosphorylation of beta-catenin by AKT promotes beta-catenin transcriptional activity. J. Biol. Chem. 282, 11221–11229 (2007).

Aliases: 20 kDa myosin light chain antibody; Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody; LC20 antibody; MGC3505 antibody; MLC 2 antibody; MLC-2C antibody; MLC2 antibody; MLY 9 antibody; MRLC1 antibody; MYL9 antibody; MYL9_HUMAN antibody; Myosin light chain 9 regulatory antibody; Myosin light polypeptide 9 regulatory antibody; myosin regulatory light chain 1 antibody; Myosin regulatory light chain 2 antibody; Myosin regulatory light chain 2 smooth muscle isoform antibody; Myosin regulatory light chain 9 antibody; Myosin regulatory light chain MRLC1 antibody; Myosin regulatory light polypeptide 9 antibody; Myosin RLC antibody; Myosin vascular smooth muscle light chain 2 antibody; MYRL2 antibody; OTTHUMP00000030857 antibody; smooth muscle isoform antibody

UniProt: P24844