c-MYC antibody (pThr358)

Catalog No. ABIN7138839

Product Details anti-c-MYC Antibody

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Binding Specificity: pThr358

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of threonine 358 (R-R-T(p)-H-N) derived from Human Myc.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: MYC (MYC Products)

Background:

Background: Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.

Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702.
Blackwood E M, et al. (1991) Science. 251:1211-1217.
Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.
Grandori C, et al. (2000) Annu Rev Cell Dev Biol. 16: 653-699.

Aliases: AU016757 antibody, Avian myelocytomatosis viral oncogene homolog antibody, bHLHe39 antibody, c Myc antibody, Cellular myelocytomatosis oncogene antibody, Class E basic helix-loop-helix protein 39 antibody, MGC105490 antibody, MRTL antibody, Myc antibody, Myc protein antibody, Myc proto oncogene protein antibody, Myc proto-oncogene protein antibody, myc-related translation/localization regulatory factor antibody, MYC_HUMAN antibody, Myc2 antibody, myca antibody, MYCC antibody, Myelocytomatosis oncogene a antibody, Myelocytomatosis oncogene antibody, Niard antibody, Nird antibody, oncogene c-Myc antibody, Oncogene Myc antibody, OTTHUMP00000158589 antibody, OTTHUMP00000227763 antibody, Proto-oncogene c-Myc antibody, Protooncogene homologous to myelocytomatosis virus antibody, RNCMYC antibody, Transcription factor p64 antibody, Transcriptional regulator Myc-A antibody, V-Myc avian myelocytomatosis viral oncogene homolog antibody, v-myc myelocytomatosis viral oncogene homolog (avian) antibody, zc-myc antibody

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect